Production of oxygen sensor with acrylamide gel-immobilized photobacteria
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چکیده
منابع مشابه
Concentrating acrylamide gel spots.
INTRODUCTIONThis protocol describes a method for concentrating gel bands or spots excised from 1D or 2D acrylamide gels. Several replicate protein bands (or spots) are often required to provide sufficient material for sequencing. In these situations, it is necessary to excise several stained protein bands or spots from the gels and pre-concentrate them prior to electroblotting for amino-termina...
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M.F. Desimone a,1, M.C. De Marzi b,1, G.J. Copello a, M.M. Fernández b, F.L. Pieckenstain c, E.L. Malchiodi b, L.E. Diaz a,∗ a Cátedra de Quı́mica Analı́tica Instrumental, Facultad de Farmacia y Bioquı́mica, Universidad de Buenos Aires, Junı́n 956 Piso 3◦ (1113) Ciudad de Buenos Aires, Argentina b Cátedra de Inmunologı́a, IDEHU-CONICET, Facultad de Farmacia y Bioquı́mica, Universidad de Buenos Aires,...
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A thin-layer acrylamide gel electrophoresis technique suitable for analysis of serum or urine proteins is described in detail. The method gives sharp differentiation of protein fractions and is particularly suitable for the analysis of biological solutions containing proteins in low concentration.
متن کاملHemoglobin electrophoresis in acrylamide gel.
By THOMAS G. FERRIS, ROBERT E. EASTERLING AND RICHARD E. BUDD T HE VARIOUS types of hemoglobins have been identified and reported by means of zone electrophoresis using supporting media such as filter paper,’ starch,2 agar,3 cellulose acetate,4 and combinations of these media. Recently a synthetic acrylamide gel ( Cyanogum 41#{176} ) has been investigated and found to have characteristics which...
متن کاملBacitracin production by whole cells immobilized in polyacrylamide gel.
Whole cells of Bacillus sp., bacitracin-producing bacteria, were immobilized in polyacrylamide gel prepared by using 5% total acrylamide (95% acrylamide monomer and 5% N, N'-methylenebis acrylamide). Bacitracin production by the immobilized whole cells was examined by using various fermentation media. In starch-bouillon medium, the initial activity of immobilized whole cells for bacitracin prod...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1983
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.32.3_159